Last November the supermarket got the ball rolling by scrapping these boxes for its own brand toothpaste – a move which will lead to the saving of more than 55 tonnes of cardboard each year.
The new trial will take place in nearly 30 Tesco stores across the UK and will include all the major toothpaste brands such as Colgate, Oral B, Sensodyne, Aquafresh and Corsadyl.
If successful, box-less toothpaste will then be rolled out across other Tesco stores, with a potential saving of 680 tonnes of cardboard a year.
Tesco Oral Care Buyer Felicity Bexton said: "We made a bold move to remove un-needed toothpaste boxes last November on our own brand and have had positive feedback from customers. Now we are working with the major toothpaste brands to join us on this journey.
"Not only is there an opportunity to remove this needless packaging, but it also means being able to transport more tubes in the same amount of space, helping us take delivery lorries off the road too.
"We think that for customers the move makes sense, because the first thing they do when they buy toothpaste is throw the packaging box away!"
Tesco will sell tubes loose, free from the outer cardboard box.
This move comes as part of Tesco's 4Rs packaging strategy to tackle the impact of packaging waste. Since the launch of the 4Rs strategy in August 2019, the supermarket compny claims it has reduced packaging by more than 3,000 tonnes from its annual footprint.